SF
| Pos | No | Player 1 | No | Player 2 | No | Player 3 | No | Player 4 | No | Player 5 |
|---|---|---|---|---|---|---|---|---|---|---|
| Offense | ||||||||||
| LWR | 11 | Aiyuk, Brandon 20/1 | 13 | James, Richie 18/7 | 86 | Cracraft, River SF20 | 82 | Cole, Matt P/Mia | ||
| RWR | 19 | Samuel, Deebo 19/2 | 84 | Bourne, Kendrick CF17 | 15 | Taylor, Trent 17/5 | ||||
| LT | 67 | Skule, Justin 19/6 | 68 | McKivitz, Colton 20/5 | ||||||
| LG | 75 | Tomlinson, Laken T/Det | 66 | COMPTON, TOM U/NYJ | ||||||
| C | 60 | Brunskill, Daniel SF19 | 50 | Grasu, Hroniss SF20 | ||||||
| RG | 62 | BERGSTROM, TONY SF20 | ||||||||
| RT | 69 | McGlinchey, Mike 18/1 | ||||||||
| TE | 85 | Kittle, George 17/5 | 89 | Woerner, Charlie 20/6 | ||||||
| TE | 81 | REED, JORDAN SF20 | 82 | Dwelley, Ross CF18 | ||||||
| QB | 3 | Beathard, CJ 17/3 | 2 | Rosen, Josh P/TB | ||||||
| RB | 30 | Wilson, Jeffery CF18 | 28 | McKinnon, Jerick U/Min | 26 | Coleman, Tevin U/Atl | 48 | Walter, Austin CF19 | ||
| FB | 44 | Juszczyk, Kyle U/Bal | ||||||||
| Defense | ||||||||||
| LDE | 92 | Hyder, Kerry U/Dal | 96 | JORDAN, DION SF20 | ||||||
| LDT | 99 | Kinlaw, Javon 20/1 | 90 | Givens, Kevin CF19 | 65 | Daniels, Darrion CF20 | ||||
| RDT | 93 | Jones, DJ 17/6 | 95 | Street, Kentavius 18/4 | ||||||
| RDE | 91 | Armstead, Arik 15/1 | 64 | Barrett, Alex SF19 | ||||||
| SAM | 57 | Greenlaw, Dre 19/5 | 53 | NZEOCHA, MARK P/Dal | ||||||
| MIKE | 54 | Warner, Fred 18/3 | 51 | Al-Shaair, Azeez CF19 | ||||||
| WILL | 45 | FLANNIGAN-FOWLES, DEMETRIUS CF19 | 59 | Walker, Joe U/Arz | ||||||
| LCB | 22 | Verrett, Jason U/LAC | 41 | Moseley, Emmanuel CF18 | ||||||
| SS | 33 | Moore, Tarvarius 18/3 | 36 | Harris, Marcell 18/6 | 48 | Nacua, Kai SF20 | ||||
| FS | 20 | Ward, Jimmie 14/1 | 33 | Moore, Tarvarius 18/3 | ||||||
| RCB | 25 | SHERMAN, RICHARD CC/Sea | 23 | Witherspoon, Ahkello 17/3 | ||||||
| NB | 27 | Johnson, Dontae 14/4 | ||||||||
| Special Teams | ||||||||||
| PT | 6 | Wishnowsky, Mitch 19/4 | ||||||||
| PK | 9 | GOULD, ROBBIE U/NYG | ||||||||
| LS | ||||||||||
| H | 6 | Wishnowsky, Mitch 19/4 | ||||||||
| KO | 9 | GOULD, ROBBIE U/NYG | ||||||||
| PR | 86 | Cracraft, River SF20 | ||||||||
| KR | 28 | McKinnon, Jerick U/Min | ||||||||
| Reserves | ||||||||||
| OpOut | 17 | BENJAMIN, TRAVIS VolOp | 64 | Brendel, Jake VolOp | 78 | Coleman, Shon VolOp | ||||
| SUS | 24 | Williams, K'Waun SUS | ||||||||
| RES | 14 | Hurd, Jalen IR | 2 | Thompson, Chris IR | 10 | Garoppolo, Jimmy IR/DFR | 40 | Webster, Ken IR | 78 | Willis, Jordan IR |
| RES | 98 | Blair, Ronald PUP | 58 | Richburg, Weston PUP | 47 | TAYLOR, JAMAR IR | 55 | Ford, Dee IR | 71 | WILLIAMS, TRENT IR |
| RES | 97 | Bosa, Nick IR | 94 | Thomas, Solomon IR | 63 | GARLAND, BEN IR | 4 | Mullens, Nick IR | 98 | ANSAH, ZIGGY IR |
| RES | 31 | Mostert, Raheem IR | 46 | Pepper, Taybor Covid-19 | 29 | Tartt, Jaquiski IR | 38 | Hasty, JaMycal IR | ||
| FUT | 00 | Alexander, Adonis FUT/CB | 76 | Coatney, Josiah FUT/DT | 38 | Edwards, Chris FUT/S | 47 | Griffith, Jonas FUT/IB | 77 | Hall, Daeshon FUT/DE |
| FUT | 35 | Harris, Tim FUT/CB | 40 | Hokit, Josh FUT/B | 17 | Jennings, Jauan FUT/WR | 00 | Melifonwu, Obi FUT/FS | 00 | Proehl, Austin FUT/WR |
| FUT | 61 | Shepley, Dakoda FUT/OT | 88 | White, Kevin FUT/WR | 76 | Williams, Isaiah FUT/OT | 83 | Harrell, Chase FUT/TE | 43 | Mayden, Jared FUT/SS |
| FUT | 00 | JOHNSON, JOSH FUT/QB | 00 | Kaufusi, Corbin FUT/DE | ||||||