SF
| Pos | No | Player 1 | No | Player 2 | No | Player 3 | No | Player 4 | No | Player 5 |
|---|---|---|---|---|---|---|---|---|---|---|
| Offense | ||||||||||
| LWR | 11 | Aiyuk, Brandon 20/1 | 13 | James, Richie 18/7 | 00 | Sherfield, Trent R/Arz | 86 | Cracraft, River SF20 | 82 | Cole, Matt P/Mia |
| LWR | 88 | White, Kevin SF20 | ||||||||
| RWR | 19 | Samuel, Deebo 19/2 | 14 | SANU, MOHAMED U/Det | 14 | Hurd, Jalen 19/3 | 17 | Jennings, Jauan 20/7 | 00 | Proehl, Austin SF21 |
| RWR | 17 | BENJAMIN, TRAVIS U/LAC | ||||||||
| LT | 71 | WILLIAMS, TRENT T/Was | 78 | Coleman, Shon T/Cle | ||||||
| LG | 75 | Tomlinson, Laken T/Det | ||||||||
| C | 00 | MACK, ALEX U/Atl | 58 | Richburg, Weston U/NYG | 64 | Brendel, Jake SF20 | ||||
| RG | 67 | Skule, Justin 19/6 | 60 | Brunskill, Daniel SF19 | 76 | Williams, Isaiah SF20 | ||||
| RT | 69 | McGlinchey, Mike 18/1 | 68 | McKivitz, Colton 20/5 | 61 | Shepley, Dakoda SF20 | ||||
| TE | 85 | Kittle, George 17/5 | 89 | Woerner, Charlie 20/6 | 83 | Harrell, Chase CF20 | 82 | Dwelley, Ross CF18 | ||
| QB | 10 | Garoppolo, Jimmy T/NE | 4 | Mullens, Nick CF17 | 2 | Rosen, Josh P/TB | 1 | JOHNSON, JOSH SF20 | 00 | Sudfeld, Nate U/Phi |
| RB | 30 | Wilson, Jeffery CF18 | 31 | Mostert, Raheem SF16 | 38 | Hasty, JaMycal CF20 | 48 | Walter, Austin CF19 | 40 | Hokit, Josh CF20 |
| FB | 44 | Juszczyk, Kyle U/Bal | 88 | Helm, Daniel P/TB | ||||||
| Defense | ||||||||||
| LDE | 91 | Armstead, Arik 15/1 | 55 | FORD, DEE T/KC | 78 | Willis, Jordan W/NYJ | 77 | Hall, Daeshon SF20 | ||
| LDT | 00 | KERR, ZACH CC/Car | 90 | Givens, Kevin CF19 | 65 | Daniels, Darrion CF20 | ||||
| RDT | 99 | Kinlaw, Javon 20/1 | 93 | Jones, DJ 17/6 | 76 | Coatney, Josiah SF20 | ||||
| RDE | 97 | Bosa, Nick 19/1 | 95 | Street, Kentavius 18/4 | 64 | Barrett, Alex SF19 | 00 | Kaufusi, Corbin SF21 | ||
| SAM | 57 | Greenlaw, Dre 19/5 | 53 | NZEOCHA, MARK P/Dal | ||||||
| MIKE | 54 | Warner, Fred 18/3 | 51 | Al-Shaair, Azeez CF19 | 47 | Griffith, Jonas CF20 | ||||
| WILL | 45 | FLANNIGAN-FOWLES, DEMETRIUS CF19 | 50 | Ebukam, Samson U/LAR | 00 | Gerry, Nathan U/Phi | ||||
| LCB | 22 | Verrett, Jason U/LAC | 41 | Moseley, Emmanuel CF18 | 00 | Alexander, Adonis SF21 | 00 | Fields, Mark W/Hou | ||
| SS | 29 | Tartt, Jaquiski 15/2 | 36 | Harris, Marcell 18/6 | 00 | WILSON, TAVON U/Ind | 48 | Nacua, Kai SF20 | 43 | Mayden, Jared CF20 |
| FS | 20 | Ward, Jimmie 14/1 | 33 | Moore, Tarvarius 18/3 | 38 | Edwards, Chris SF20 | 00 | Melifonwu, Obi SF21 | ||
| RCB | 27 | Johnson, Dontae 14/4 | 47 | Boddy-Calhoun, Briean SF20 | 40 | Webster, Ken P/Mia | 35 | Harris, Tim 19/6 | ||
| NB | 24 | Williams, K'Waun SF17 | ||||||||
| Special Teams | ||||||||||
| PT | 6 | Wishnowsky, Mitch 19/4 | ||||||||
| PK | 9 | GOULD, ROBBIE U/NYG | ||||||||
| LS | 46 | Pepper, Taybor SF20 | ||||||||
| H | 6 | Wishnowsky, Mitch 19/4 | ||||||||
| KO | 9 | GOULD, ROBBIE U/NYG | ||||||||
| PR | 86 | Cracraft, River SF20 | ||||||||
| KR | ||||||||||
| Free Agents / Cap Casualties | ||||||||||
| FACC | 13 | GOODWIN, MARQUISE CC | 98 | ANSAH, ZIGGY UFA | 62 | BERGSTROM, TONY UFA | 66 | COMPTON, TOM UFA | 63 | GARLAND, BEN UFA |
| FACC | 50 | Grasu, Hroniss UFA | 96 | JORDAN, DION UFA | 28 | McKinnon, Jerick UFA | 81 | REED, JORDAN UFA | 25 | SHERMAN, RICHARD UFA |
| FACC | 59 | Walker, Joe UFA | 47 | TAYLOR, JAMAR UFA | 15 | Taylor, Trent UFA | ||||